Journal: Discover Oncology
Article Title: CTHRC1 modulates cell proliferation and invasion in hepatocellular carcinoma by DNA methylation
doi: 10.1007/s12672-024-01194-8
Figure Lengend Snippet: Expression of CTHRC1 correlates with DNA methylation levels in HCC cell lines. A The methylation levels of CTHRC1 in the promoter region were detected by Agena MassARRAY® Methylation in HSC cell line LX-2 and three HCC cell lines HepG2, Huh-7, and Hep3B2.1 (left), and presented in hot map (right). B RT-qPCR and C western bolt were used to detect the mRNA and protein levels of CTHRC1 in LX-2, HepG2, Huh-7, and Hep3B2.1 cell lines. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 vs. LX-2 group. D RT-qPCR was used to detect the mRNA levels of CTHRC1 in LX-2, HepG2, Huh-7, and Hep3B2.1 cell lines treated with 5-Aza-DC. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001
Article Snippet: Agena MassARRAY® Methylation Analysis assessed the methylation level of CTHRC1 in the promoter region.
Techniques: Expressing, DNA Methylation Assay, Methylation, Quantitative RT-PCR, Western Blot